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Plasmids of Ketogulonicigenium robustum - strains NRRL B-21627 and SPU_B003
The DNA sequences of four plasmids carried by Ketogulonicigenium robustum strain SPU_B003 are virtually identical to those of four plasmids carried by K. robustum type strain ADM X6L (NRRL B-21627). The specifics are presented in this technical note.
While monitoring the CO2 level within a newly installed vinyl anaerobic chamber, I noticed a steady loss of CO2 over a period of days that could only have been caused by diffusion of CO2 through the vinyl. This prompted me to model chamber operations over time to better understand the combined effect of CO2 loss from diffusion and CO2 gain from airlock operation. Here I review some chamber basics, show outcomes of the model, review why CO2 is used in anaerobic chambers, and describe how I monitor and control CO2.
A description of 127 surviving bacterial strain stocks once owned by professor Elizabeth McCoy of the University of Wisconsin, including many strains not in public collections, is presented. The stocks are primarily spores in soil and are accompanied by a strain catalog consisting of 231 hand-written records. A 1985 letter about the strains written by Dr. Leland McClung of Indiana University is also included.
A comprehensive list of papers and dissertations from the University of Wisconsin between 1923 and 1985 about butanol- and butyric acid bacteria, is herein presented.
Two fine paintings, "Memories of clostridia" and "Pancake Bunny", created in acrylic by Dr. Eva R. Kashket are presented.
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Bacteriophage particles propagated on Clostridium beijerinckii NCIMB 8052 as the host strain are shown in the upper-right TEM image of this web page. The negative stain is 1% uranyl acetate. The phage was isolated in the lab of Dr. Thomas M. Schmidt of the University of Michigan. The capsid diameter is about 60 nanometers. The image is displayed with Dr. Schmidt's permission.
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