BLAST alignments of Ketogulonicigenium robustum SPU_B003 plasmids 
against Ketogulonicigenium robustum NRRL B-21627 (ADM X6L) plasmids.

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NCBI BLAST DATABASE SEARCHED:

   Database: Nucleotide sequences derived from the Patent division of GenBank
   	   45,782,177 sequences; 25,558,976,770 total bases
   Date: Nov 5, 2021  8:54 PM	Longest sequence: 33,862,096 bases
   BLASTDB Version: 5


HITS THAT WERE DELETED TO SIMPLIFY THIS DISPLAY:

   For each of the blast results shown below, only the alignment to the first-hit target 
   sequence is shown in order to simplify this display.  The deleted target sequences are 
   duplicates that entered the NCBI patnt database as a result of divisional patents that 
   issued under the specification of the original application, plus a PCT application. 
   The issued patents accounting for the sequence redundancy in the patnt database are 
   US 7030233, US 7053196, US 7053197 and WO0177348.

FASTA HEADERS OF STRAIN SPU_B003 PLASMIDS:

   >NZ_CP019939.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed2, complete sequence
   >NZ_CP019940.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed3, complete sequence
   >NZ_CP019941.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed4, complete sequence
   >NZ_CP019942.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed5, complete sequence

   ( NZ_CP019938.1 "plasmid unnamed1" is a megaplasmid or chromid and is not part of this analysis )

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> pADMX6L3 (AR884694.1) vs. SPU_B003 plasmid 2

Comment:
- pADMX6L3 is one base pair longer than plasmid 2 of SPU_B003 but is otherwise identical
- The sequences are represented as reverse complements of one another
- The plasmids are linearized at different coordinates, which is why two alignments 
  are returned instead of one.

# BLASTN 2.12.0+
# Query: NZ_CP019939.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed2, complete sequence
# Database: ../../blast_db/patnt
# Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, 
          q. start, q. end, s. start, s. end, evalue, bit score
# 17 hits found
NZ_CP019939.1	AR884694.1	100.000	14664	0	0	5031	19694	19695	5032	0.0	27080
NZ_CP019939.1	AR884694.1	100.000	5031	0	0	1	5031	5031	1	0.0	9291

Accessions of removed (redundant) hits:
AR884687.1, AR835940.1, AX278182.1, AR884698.1, AR884691.1, AR835944.1, AX278186.1
FW306007.1, which is unrelated to the query sequence registered a short low-score hit


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> pADMX6L1 (AR884692.1) vs. SPU_B003 plasmid 3

Comment:
- pADMX6L1 is one base pair longer than plasmid 3 of SPU_B003 but is otherwise identical
- The two sequences represent the same DNA strand of the plasmid
- The plasmids are linearized at different coordinates, which is why two alignments 
  are returned instead of one.

# BLASTN 2.12.0+
# Query: NZ_CP019940.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed3, complete sequence
# Database: ../../blast_db/patnt
# Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, 
          q. start, q. end, s. start, s. end, evalue, bit score
# 16 hits found
NZ_CP019940.1	AR884692.1	100.000	4775	0	0	1	4775	2255	7029	0.0	8818
NZ_CP019940.1	AR884692.1	100.000	2254	0	0	4775	7028	1	2254	0.0	4163

Accessions of removed (redundant) hits:
AR884685.1, AR835938.1, AX278180.1, AR884696.1, AR884689.1, AR835942.1, AX278184.1


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> pADMX6L2 (AR884693.1) vs. SPU_B003 plasmid 4

Comment:
- The pADMX6L2 and SPU_B003 plasmid 4 sequences are identical
- The sequences are represented as reverse complements of one another
- The plasmids are linearized at different coordinates, which is why two alignments 
  are returned instead of one.

# BLASTN 2.12.0+
# Query: NZ_CP019941.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed4, complete sequence
# Database: ../../blast_db/patnt
# Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, 
          q. start, q. end, s. start, s. end, evalue, bit score
# 16 hits found
NZ_CP019941.1	AR884693.1	100.000	3960	0	0	1	3960	3960	1	0.0	7313
NZ_CP019941.1	AR884693.1	100.000	45	0	0	3961	4005	4005	3961	4.23e-12	84.2

Accessions of removed (redundant) hits:
AR884686.1, AR835939.1, AX278181.1, AR884697.1, AR884690.1, AR835943.1, AX278185.1


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> pADMX6L4 (AR884695.1) vs. SPU_B003 plasmid 5

Comment:
- pADMX6L4 and SPU_B003 plasmid 5 sequences differ by only a single base pair
  ( for perfect identity, coordinate #2523 of AR884695.1 would change from 'G' to 'A' )
- The sequences are represented as reverse complements of one another
- The plasmids are linearized at different coordinates, which is why two alignments 
  are returned instead of one.

# BLASTN 2.12.0+
# Query: NZ_CP019942.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed5, complete sequence
# Database: ../../blast_db/patnt
# Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, 
          q. start, q. end, s. start, s. end, evalue, bit score
# 8 hits found
NZ_CP019942.1	AR884695.1	100.000	2176	0	0	1	2176	2176	1	0.0	4019
NZ_CP019942.1	AR884695.1	99.951	2035	1	0	2177	4211	4211	2177	0.0	3753

Accessions of removed (redundant) hits:
AR884688.1, AR835941.1, AX278183.1


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