BLAST alignments of Ketogulonicigenium robustum SPU_B003 plasmids against Ketogulonicigenium robustum NRRL B-21627 (ADM X6L) plasmids. ================================================================================ NCBI BLAST DATABASE SEARCHED: Database: Nucleotide sequences derived from the Patent division of GenBank 45,782,177 sequences; 25,558,976,770 total bases Date: Nov 5, 2021 8:54 PM Longest sequence: 33,862,096 bases BLASTDB Version: 5 HITS THAT WERE DELETED TO SIMPLIFY THIS DISPLAY: For each of the blast results shown below, only the alignment to the first-hit target sequence is shown in order to simplify this display. The deleted target sequences are duplicates that entered the NCBI patnt database as a result of divisional patents that issued under the specification of the original application, plus a PCT application. The issued patents accounting for the sequence redundancy in the patnt database are US 7030233, US 7053196, US 7053197 and WO0177348. FASTA HEADERS OF STRAIN SPU_B003 PLASMIDS: >NZ_CP019939.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed2, complete sequence >NZ_CP019940.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed3, complete sequence >NZ_CP019941.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed4, complete sequence >NZ_CP019942.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed5, complete sequence ( NZ_CP019938.1 "plasmid unnamed1" is a megaplasmid or chromid and is not part of this analysis ) ================================================================================ ================================================================================ > pADMX6L3 (AR884694.1) vs. SPU_B003 plasmid 2 Comment: - pADMX6L3 is one base pair longer than plasmid 2 of SPU_B003 but is otherwise identical - The sequences are represented as reverse complements of one another - The plasmids are linearized at different coordinates, which is why two alignments are returned instead of one. # BLASTN 2.12.0+ # Query: NZ_CP019939.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed2, complete sequence # Database: ../../blast_db/patnt # Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, q. start, q. end, s. start, s. end, evalue, bit score # 17 hits found NZ_CP019939.1 AR884694.1 100.000 14664 0 0 5031 19694 19695 5032 0.0 27080 NZ_CP019939.1 AR884694.1 100.000 5031 0 0 1 5031 5031 1 0.0 9291 Accessions of removed (redundant) hits: AR884687.1, AR835940.1, AX278182.1, AR884698.1, AR884691.1, AR835944.1, AX278186.1 FW306007.1, which is unrelated to the query sequence registered a short low-score hit ================================================================================ > pADMX6L1 (AR884692.1) vs. SPU_B003 plasmid 3 Comment: - pADMX6L1 is one base pair longer than plasmid 3 of SPU_B003 but is otherwise identical - The two sequences represent the same DNA strand of the plasmid - The plasmids are linearized at different coordinates, which is why two alignments are returned instead of one. # BLASTN 2.12.0+ # Query: NZ_CP019940.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed3, complete sequence # Database: ../../blast_db/patnt # Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, q. start, q. end, s. start, s. end, evalue, bit score # 16 hits found NZ_CP019940.1 AR884692.1 100.000 4775 0 0 1 4775 2255 7029 0.0 8818 NZ_CP019940.1 AR884692.1 100.000 2254 0 0 4775 7028 1 2254 0.0 4163 Accessions of removed (redundant) hits: AR884685.1, AR835938.1, AX278180.1, AR884696.1, AR884689.1, AR835942.1, AX278184.1 ================================================================================ > pADMX6L2 (AR884693.1) vs. SPU_B003 plasmid 4 Comment: - The pADMX6L2 and SPU_B003 plasmid 4 sequences are identical - The sequences are represented as reverse complements of one another - The plasmids are linearized at different coordinates, which is why two alignments are returned instead of one. # BLASTN 2.12.0+ # Query: NZ_CP019941.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed4, complete sequence # Database: ../../blast_db/patnt # Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, q. start, q. end, s. start, s. end, evalue, bit score # 16 hits found NZ_CP019941.1 AR884693.1 100.000 3960 0 0 1 3960 3960 1 0.0 7313 NZ_CP019941.1 AR884693.1 100.000 45 0 0 3961 4005 4005 3961 4.23e-12 84.2 Accessions of removed (redundant) hits: AR884686.1, AR835939.1, AX278181.1, AR884697.1, AR884690.1, AR835943.1, AX278185.1 ================================================================================ > pADMX6L4 (AR884695.1) vs. SPU_B003 plasmid 5 Comment: - pADMX6L4 and SPU_B003 plasmid 5 sequences differ by only a single base pair ( for perfect identity, coordinate #2523 of AR884695.1 would change from 'G' to 'A' ) - The sequences are represented as reverse complements of one another - The plasmids are linearized at different coordinates, which is why two alignments are returned instead of one. # BLASTN 2.12.0+ # Query: NZ_CP019942.1 Ketogulonicigenium robustum strain SPU_B003 plasmid unnamed5, complete sequence # Database: ../../blast_db/patnt # Fields: query acc.ver, subject acc.ver, % identity, alignment length, mismatches, gap opens, q. start, q. end, s. start, s. end, evalue, bit score # 8 hits found NZ_CP019942.1 AR884695.1 100.000 2176 0 0 1 2176 2176 1 0.0 4019 NZ_CP019942.1 AR884695.1 99.951 2035 1 0 2177 4211 4211 2177 0.0 3753 Accessions of removed (redundant) hits: AR884688.1, AR835941.1, AX278183.1 ================================================================================